PETA is calling for an investigation of the Mobile Zoo in Wilmer.
The animal rights group has filed complaints with the State of Alabama and the federal government.
PETA's concerns surround threatened tortoises cages they say the zoo is holding without proper permits.
The group also said the zoo's cages violate the Endangered Species Act.
A previous complaint from PETA launched an investigation into the care and caging of the big cats by the U.S. Department of Agriculture.